The optimal water source for koi is natural mountain spring or stream water that is guaranteed to be safe and unpolluted, as it is typically rich in dissolved oxygen, low in contaminants, and stable in temperature. However, such an ideal source is often difficult to obtain reliably.
A more practical and commonly used alternative is deep well water, which is usually clean and stable, though its hardness and mineral content should be checked. Another widely accessible and safe option is municipal tap water that has been properly dechlorinated (aged for 24-48 hours or treated with a water conditioner).

It is generally not recommended to use surface water (such as from rivers, lakes, or ponds) or shallow groundwater due to the high risks of contamination, the presence of pathogens, significant seasonal fluctuations in quality, and potential agricultural or industrial pollutant runoff. Regardless of the source chosen, it is crucial to test key parameters—such as pH, hardness, ammonia, nitrite, and nitrate—before introducing the water to the koi pond and to ensure all water used is free of chlorine, chloramines, and heavy metals.